The name of enzyme involved in this experiment is salivary amylase. which presents in our spit. Salivary amylase catalyses the dislocation of complex sugar. such as amylum. into a simple sugar. such as glucose. In this experiment. the optimal temperature for salivary amylase to map is 37?C. Above the optimal temperature. the kinetic energy in the substrate and enzyme additions.
The figure of hits between substrate and enzyme besides increases. but the formation of enzyme-substrate complex lessenings. This is because the increasing of kinetic energy causes the amino acid molecules to vibrate violently. Some bonds like H and ionic bonds which hold the constellation of the enzyme interruption. The form of active site of the enzyme alterations and is non able to adhere with the substrate any longer. 3D construction of the enzyme is said to be destroyed. Therefore. the enzyme denatures at high temperature and loses its catalytic activity for good.
Hydrochloric acid. HCL in this experiment is used to hydrolyse the composite sugar which nowadays in solution B into monomers or simpler sugars. At 37?C. salivary amylase nowadays in saliva reacts actively to hydrolyse the complex sugar in solution B as 37?C is its optimal temperature. Therefore. it gives positive consequence for the Benedict’s trial. which is formed brick-red precipitate. However. at the same temperature. hydrochloric acerb gives negative consequence as it is unable to hydrolyse the content in solution B. At 95?C. hydrochloric acid has obtained adequate activation energy and interrupt the bonds of complex sugar in solution B into simpler sugars.
Therefore. it produces positive consequence for Benedict’s trial. Inversely. salivary amylase is denatured at 95?C and unable to respond usually with substrate in solution B and hence. the consequence for Benedict’s trial is negative. The merchandise formed when 10ml of solution B and 1ml of saliva react at 37?C is monosaccharide. The merchandise formed when 10ml of solution B and 1ml of 3M hydrochloric acid react at 95?C is monosaccharide excessively. Solution A gives positive consequence for Benedict’s trial. which indicates it is one of the cut downing sugars and the negative consequence for iodine trial shows that amylum is absent in solution A. In solution B. cut downing sugar is absent because the consequence for Benedict’s trial is negative. However. the consequence for iodine trial is positive. which indicates amylum is present in solution B.
In trial tubings 1 and 3. cut downing sugar is present after reacted in 37?C and 95?C severally because it show positive consequence when tested with Benedict’s reagent. So. the construction of the sugar in solution A is simpler. while the construction of the sugar in solution B is more complex as it needs salivary amylase or hydrochloric acid to hydrolyse before it gives positive consequence for Benedict’s trial.
Iodine trial is used to observe the presence of amylum in a solution. When amylum nowadayss. iodine changes its coloring material from xanthous to dark purple. Benedict’s trial is used to observe the presence of cut downing sugar in a solution. When cut downing sugar nowadayss. a brick-red precipitate appears in the solution. Benedict reagent contains Cu ( II ) ions. which is bluish in coloring material can be reduced to Cu ( I ) ions. which is indissoluble brick-red precipitate when cut downing sugar is present. Carbohydrate B is more complex than saccharide A. because salivary amylase or hydrochloric acid is needed at 37?C and 95?C severally to be hydrolyzed into monomers before it gives positive consequence for Benedict’s trial. In decision. solution A contains simple sugar and solution B contains amylum. Salivary amylase actives at 37?C. while hydrochloric acid actives at 95?C.
