Investigating the consequence of java on the bosom rate of water flea Aim:
To happen out the consequence of different caffeine concentrations on bosom rate in water flea Independent variable:
•Caffeine concentration
Dependent variable:
•Daphnia bosom rate
Controlled variables:
•Size of water flea
•Habitat from which the water flea is obtained
•Temperature of the surrounding
How the variables can be controlled:
•Equally sized water flea should be used in the experiment
•All water flea should be collected at a same topographic point. in uncontaminated Waterss •Experiment should be carried out in a lab at room temperature Apparatus/materials:
•Pipette
•Petri dish
•Filter paper
•Silicon lubricating oil
•Needle
•0. 1 % . 0. 2 % . 0. 3 % . 0. 4 % . 0. 5 % of caffeine solutions
•Daphnia civilization
Methodology:
•Select a big specimen and. with a pipette. reassign it to the Centre of a little. dry petri dish. with filter paper take extra H2O from around the specimen so that it is wholly stranded •With a searcher topographic point a little blob of silicone lubricating oil onto the floor of the petri dish. Then wipe the needle clean and utilize it to gently force the posterior terminal of the animate being into the lubricating oil so that it is steadfastly anchored. Now fill the petri dish with a caffeine solution. •Place the petri dish on the phase of a microscope and detect the carnal under low power and watch the bosom crushing •Repeat the experiment with the remainder of the caffeine solutions Consequences:

Caffeine concentration/ % Heart rate/Beats per 2 proceedingss
Trial 1Trial 2Trial 3Trial 4Trial 5AverageAverage beats/min
0. 1258256279306295279140
0. 2380390390380382384192
0. 3389398404410400400200
0. 4267440410440428397199
0. 5436419443450435437219

Decision:
In decision the informations collected supports that an addition in the caffeine concentration leads to an addition in the bosom rate of the water flea