Throughout this study you will derive information as to how temperature effects the sum of O produced in an enzyme- catalase experiment. In the experiment we used liver extract as a catalase and created a chemical reaction within a reaction chamber between the catalase and H peroxide every bit good as three different controlled temperatures. In the process below there will be a measure by measure procedure as to how the experiment was created. As a consequence we found that the higher the temperature in the H2O bath the more O will be produced and “rejected” from the reaction chamber. In decision. the reaction chamber placed in the highest temperature H2O bath produced an norm of. 5 more millilitres of O than the reaction chamber placed in the lowest temperature H2O bath tested. While making scientific research antecedently in the hebdomad. pupils found information about enzymes and how they work. In the concentration of catalase lab pupils found that the less enzyme concentration nowadays. the less O is produced. Harmonizing to Science. com chemical reactions occur when two or more compounds collide. A chemical reaction occurs when any two substances come together to organize one.

In the first enzyme experiment covering with concentration when the catalase discs came in contact with the H peroxide it created a chemical reaction which in bend caused O to be produced. When the concentration of the catalase discs were reduced ( diminish the figure of discs in the experiment ) less O was produced because the H peroxide had less catalase to respond with. hence the ground for reduced sum of O in millilitres at the terminal of the five minute experimentation clip period. The end for the original ( concentration ) experiment was to see how the concentration degrees of the catalase effected the sum of O produced. That was merely a basal line for what was to go on next. As a category we divided and conquered to delve deeper into a more controlled experiments to calculate out what truly was set uping the sum of O produced. My group focused on temperature. If the temperature of the H2O bath that the reaction chamber is placed in is increased so the sum of O produced will increase. The independent variable is the temperature of the H2O and the dependant variable is the sum of O that is produced. ( In millilitres )

Here is an illustration of how I would hold written a lab process for other people trying the experiment. Materials:

• 4. 50mL beaker’s. with 1 incorporating fresh catalase solution
• Reaction chamber
• Filter paper discs
• Forcepss
• Ring base & A ; clinch
• 10mL graduated cylinder
• 100mL graduated cylinder
• 3 % H peroxide ( H2O2 )
• pan ( H2O bath )
• Hot home base ( along with heat protectant baseball mitts )
• Ice
• Thermometer
• distilled H2O
Procedure:
1. Work as a lab group of 3-4 members. Each lab group will finish Part A of the lab and repetition.

2. At your lab tabular array you will happen a square bottle with a medicine dropper top. This is called the Reaction chamber. You will besides happen a 100mL calibrated cylinder. pealing base and clinch. and a Plastic pan which will be used as a H2O bath. All of this equipment needs to be assembled into our experimental setup. as described and illustrated below.

3. Fill the pan 3/4 full of tap H2O and ice to chill the H2O to 7 °C

4. Submerge the 100mL graduated cylinder to make full it with H2O. Turn the calibrated cylinder upside down. maintaining the unfastened terminal under H2O. so as to maintain it filled with H2O. Suspend it upside down in the clinch on the ring base. Adjust the tallness of the clinch on the ring base so the unfastened terminal of the calibrated cylinder is about 3cm above the underside of the pan. 5. Put a thermometer in the pan and record the temperature of the H2O. during Part
A of the lab. For this experiment it should be around 7 grades Celsius.

1. Put up the experimental setup as described above.
2. Obtain a little sum of stock catalase solution in a 50mL beaker. ( Put on ice to maintain catalase cool )
3. Obtain a reaction chamber and a figure of filter paper discs. ( 4 for each test )
4. Fix 4 discs for usage in the reaction chamber. Prepare each disc. one at a clip. by keeping each by its border with a brace of forceps ( pincers ) and dunking it into the stock catalase solution for a few seconds. *** Stir your catalase solution ( liver infusion ) before every disc is dipped. Remove the extra liquid from the disc by blotting the disc on a paper towel. With another paper towel dry the tip of the forceps. so it doesn’t trickle in the following measure.
5. Following. reassign the dampened discs to the top inside wall of the reaction vas. The wet filter paper discs will lodge to the side wall of the glass. Position the discs in the upper half of the reaction vas ( the half near the gap ) . Repeat this process with the other discs. puting all discs on the same surface of the reaction vas. * One individual is each group should soak and manage all discs for all experiments. In this manner. the techniques will stay similar and cardinal operations will be performed systematically. Put four catalase-soaked filter paper discs high on one interior sidewall of the reaction chamber.
6. Stand the reaction chamber unsloped and carefully add 10mL of 3 % H Peroxide ( H2O2 ) solution. Make NOT let the peroxide to touch the filter paper discs.
7. Tightly put the stopper on the chamber.
8. Recover the H2O bath and graduated cylinder. Turn the calibrated cylinder upside down into an unsloped place. maintaining its oral cavity underwater at all times.
9. Carefully place the reaction chamber and its contents on its side in the H2O bath. Make certain that the side with the discs faces upward.
10. Travel the calibrated cylinder into a place so that its oral cavity comes to lie straight over the tip of the oral cavity of the reaction chamber so that any O2 released from the reaction chamber will roll up in the calibrated cylinder. One member of the squad should keep it in this place for the continuance of the experiment.
11. Revolve the reaction chamber 180° onto its side so that the H peroxide solution comes into contact with the catalase-soaked discs. Note the clip. This is clip “zero” .
12. Measure the gas degrees in the calibrated cylinder at 30- 2nd intervals for 5 proceedingss. Record the degrees in a information tabular array of your ain design.
13. Repeat the experiment from Part A. utilizing 2 different temperatures: 27 grades Celsius and 37 grades Celsius. You may easy make this by utilizing the undermentioned processs: a. 27 grades Celsius: follow the process from Part A. but use a combination of H2O and ice to acquire the right temperature. B. 37 grades Celsius: follow the process from Part A. but use a hot home base to warm H2O to the right temperature.
14. Record all informations in a information tabular array of your ain design. Salvage information to do a graph.
15. Plot the information for Parts A and B on the same set of axes.

Throughout the experiment we made assorted observations all holding to make with the consequence that the temperature had on the sum of O being produced and coming out of the reaction chamber. For puting up the experiment we realized that keeping the coveted temperature with minimum alteration in the grade of Celsius was something that had to be maintained and watched closely. When it came to the existent experiment the 7 degree Celsius H2O bath produced less O in a clip period of 5 proceedingss increasing at a steady rate of. 1 millilitres per every 30 seconds. Overall the 27 degree Celsius H2O bath produced at a rate of. 2 millilitres per 30 seconds. Finally. the 37 degree Celsius H2O bath produced at a rate of. 5 millilitres per 30 seconds. Though the difference in O being produced per second is minimum. as the temperature increases it is clear that more O is being produced in the 5 proceedingss than when the H2O is cooler given the same sum of clip. Below is a set of informations that we collected during the experiment along with a line graph demoing our findings and equations that go along with our information. Time ( seconds )

Looking through consequences the hypothesis of “If the temperature of the H2O bath that the reaction chamber is placed in is increased so the sum of O produced will increase” was a right statement for the most portion. As temperature increased the sum of O produced increased every bit good. In the tooth-pick lab pupils posed as enzymes and did assorted things to prove what may impact the public presentation of an enzyme one of those things being temperature. In that lab pupils were asked to put their custodies in an ice bath for a certain period of clip ( 2 proceedingss I believe ) . in bend blunting our custodies ( the enzyme ) . The consequences were the same as what was predicted in our temperature experiment. the figure of tooth-picks broken was dramatically decreased after holding our custodies in the ice bath. This has direct correlativity to what we had tested in the process above. To break our experiment and acquire more accurate consequences we could hold had more control over the temperature of the H2O bath. other than that the experiment created and tested had matched our hypothesis and had sensible consequences that we could endorse up with scientific information.

The reaction chamber placed in the highest temperature H2O bath ( 37 grades Celsius ) produced an norm of. 5 more millilitres of O than the reaction chamber placed in the lowest temperature H2O bath tested ( 7 grades Celsius ) .

Citations:
Eric Hammer. Amy Carson ( August 26. 2012 ) Understanding Why Chemical Reactions are so Violent.
Retrieved From hypertext transfer protocol: //www. brighthubeducation. com/science-homework-help/ K. Hammann. S. Elliott ( October 3. 2013 ) Catalase Concentration Lab. Retrieved From room S240/ K. Hammann. S. Elliott ( October 1. 2013 ) Toothpickase Lab Activity. Retrieved From room S240/